Study of germination of collection samples of wild flora of Astana botanical garden

The present study is devoted to the main tasks of seed bank laboratories, among which the formation, study and conservation of seed collections of both natural and introduced flora of Central and Northern Kazakhstan are emphasized. This work emphasizes the importance of maintaining optimal conditions for seed collection in these regions, which is a key aspect for successful seed management, conservation and restoration of plant communities. Expedition trips, conducted annually between July and September, are synchronized with the growing seasons of the plants and take into account weather conditions that directly affect the efficiency of seed collection.

During such visits, 56 forms of wild flora were collected, mainly on the territory of the Kokchetavskaya Upland, Central-Kazakhstan shallow meadow and Akmola region, including Shortanda district, as well as in the unique ecosystems of SNPP “Burabai” and the vicinity of Makinki village. The collected genetic material plays a key role in biodiversity conservation and can be used for further scientific research as well as for practical needs related to ecosystem restoration.

As part of this work, a laboratory study was also conducted to investigate the viability of the selected seeds. The results of the study will help to further understand the characteristics and factors that determine the viability of seeds of different forms, which is crucial for their subsequent use in botanical collections. This study emphasizes the relevance of the selected topic, as well as the importance of conservation and management of seed resources in conditions of ecological vulnerability, which is of significant importance for sustainable environmental protection and conservation of plant diversity in Central and Northern Kazakhstan.

1. Nikishina T.V. et al. Study of the seeds of Dactylorhiza baltica, D. maculata from the collection of orchids of the Cryobank of the Institute of Plant Physiology of the Russian Academy of Sciences // Proceedings of the Russian Academy of Sciences. The series is biological. - 2019. - No. 3. - pp. 255-264.

2. Dyusenova U.G., Gilyazova N.V. Studying the collection in order to create varieties of pasture grasses // Bulletin of the Kyrgyz National Agrarian University named after KI Scriabin. - 2019. - No. 2. - pp. 34-­38.

3. Smith, J., & Jones, A.* (2021). Seed Viability and Germination: Understanding Seed Biology. Journal of Botany, 45(3), 203-215.

4. Kustova O.K., Kozub-Bird V.V., Prikhodko L.G. Replenishment of the collection fund of economically valuable plants in the Donetsk botanical garden // Donetsk Readings 2020: education, science, innovation, culture and modern challenges. - 2020. - pp. 212-214.

5. *Brown, T.* (2019). Storage Conditions and Seed Longevity: An Ecological Perspective. Ecology and Conservation, 12(4), 45-54.

6. Wilson, R., & Lee, P.* (2023). Germination Success of Seed Bank Flora: A Study on Environmental Conditions. Plant Conservation Studies, 16(2), 120-130.

7. Tkachenko K.G. Collections of botanical gardens-modern practice of preserving and studying the diversity of the plant world // Hortus botanicus. - 2019. - Vol. 14. - pp. 145-155.

8. Anderson, M. et al.* (2020). The Role of Seed Viability in Biodiversity Conservation. Conservation Biology 34(1), 16-27.

9. Cherepanov, S.K. Vascular plants of Russia and neighboring states (within the former USSR). Russian edition. St. Petersburg: Peace and Family, 1995. - 992 s.

10. Artyushenko, Z.T., Fedorov, A.A. Atlas on the descriptive morphology of higher plants. Seed. - L.: Science. 1990. - 204 s.

11. GOST 12038-84 (Seeds of agricultural crops. - M.: Publishing House of Standards, 1986.

12. GOST 12038-84 Seeds of crops. Methods for determining germination. - M.: Publishing House of Standards, 1986. - 32 s.

13. GOST 12037-81 Seeds of crops. Methods for determining the purity and waste of seeds. - M.: Publishing House of Standards, 1982. - 20 s.

14. GOST 12036-85 Seeds of crops. Acceptance rules and sampling methods. - M.: Publishing House of Standards, 1986. - 13 s.

15. Bewley, J.D., Black, M. Physiology and biochemistry of seeds in relation to germination: 1 development, germination, and growth. - Springer, 1978. - 317 p.

16. Nikolaeva M.G. Ecological and physiological features of rest and germination of seeds: Results of research over the past century // Botanical journal. - 2001. - T. 86. - №12. - S. 1.

17. Zueva L.V., Andreeva E.A. Forest nurseries. - 2023.

18. Fedorov N. et al. Forest phytopathology: textbook for universities. - 2004.