THE EFFECT OF PHYTOHORMONES ON CALLUSOGENESIS OF THE MEDICINAL PLANT ORIGANUM VULGARE

Abstract

Currently, the method of culturing isolated cells, tissues, and organs is widely used in research in the fields of physiology, experimental botany, genetics, biochemistry, and molecular biology. The method is used to study the regularities of cell differentiation, histo-and morphogenesis, the mechanisms of implementation of genetic information and the role of individual groups of phytohormones in these processes.
The purpose of this work is to study the effect of phytohormones on the callus culture of the plant Origanum vulgare L. First of all, sterile plants were grown in vitro from plant seeds, and those plant explants were used to obtain callus cultures. Growth regulators are 2.4-D and 6-BAP in nutrient media added in different concentrations (1.0 mg/l 2.4-D and respectively 0.5 mg/l, 1.0 mg/l, 1.5 mg/l, 2.0 mg/l, 4.0 mg/l, 5.0 mg/l, 6.0 mg/l, 9.0 mg/l 6-BAP) for growth of callus tissue and induction of callusogenesis The most effective medium was found to be MS 2 (MS + 1.0 mg/l 2.4-D + 0.5 mg/l 6-BAP). The resulting callus tissue was light and loose.