CHROMOSOMAL VARIABILITY IN POPULATIONS OF ANOPHELES MESSEAE IN THE VICINITY OF ASTANA

A cytogenetic study of Anopheles messeae Fall., 1926 natural populations was conducted in two biotopes of the Astana vicinity (Kuigenjar and International, Akmola Region). Polytene chromosomes were prepared from salivary glands of fourth-instar larvae using the lacto-aceto-orcein squash technique; 111 karyotypes were analysed. Pronounced inversion polymorphism was observed in chromosome arms XL, 3R, and 3L, whereas chromosome arm 2R was nearly monomorphic. The dominant sex-chromosome genotype was XL11; chromosome 3L showed the highest variability (3L₀₁ heterozygotes: 47.4% and 66.6%). Interpopulation differences were significant for 3R (χ²=4.37; p<0.05) and 3L (χ²=6.21; p<0.05); the International population deviated from Hardy- Weinberg equilibrium (χ²=6.87; p<0.05). The data confirm the adaptive nature of chromosomal polymorphism and local biotope differentiation within an urbanised landscape.

1. Stegniy V.N. Populyacionnaya genetika i evolyuciya malyarijnyh komarov. Tomsk: Izd – vo Tomskogo gos. un – ta. 1991. – 136 s.

2. Bismil'din F.B., SHapieva ZH.ZH., Anpilova E.N. Sovremennaya situaciya po malyarii v respublike Kazahstan // Med. parazitologiya i parazitarnye bolezni. 2001. –№ 1. – S. 24-32.

3. Gordeev M. I. Adaptivnye strategii v populyaciyah malyarijnyh komarov: dis. doktor biologicheskih nauk: 03.00.15 - Genetika. Tomsk. – 1997. – 304 s.

4. Novikov Yu.M. Vzaimodejstvie inversij v populyaciyah malyarijnogo komara Anopheles messeae Fall., differenciaciya ego kariofonda i mekhanizmy podderzhaniya populyacionno-geneticheskogo gomeostaza // V kn.: Novye dannye po kariosistematike dvukrylyh nasekomyh / Trudy Zool. in-ta AN SSSR. L., 1980, – T.95. – s. 40-49.

5. Stegniy V.N., Pishchelko A.O., Sibataev A.K., Abylkassymova G. Spatiotemporal changes in the frequencies of chromosomal inversions across the range of the malaria mosquito Anopheles messeae Fall. (Culicidae) during a 40-year monitoring period // Russian journal of Genetics. — 2016. — Vol. 52, No. 6. — P. 664–671. https://doi.org/10.7868/S0016675816060138

6. Sibataev A.K., Wasserlauf I.E., Usov K.E., Alekseeva S.S., Abylkassymova G.M., Stegniy V.N. Long-term and seasonal dynamics of inversion polymorphism in the malaria mosquito Anopheles messeae Fall. (Diptera: Culicidae) from the population of the central part of the species range (Western Siberia, Tomsk oblast) // Russian journal of Genetics. — 2023. — Vol. 59, No. 2. — P. 176–183. https://doi.org/10.1134/s1022795423020126

7. Rusakova A.M., Artemov G.N., Stegniy V.N. Inversion polymorphism of natural populations of the malaria mosquito Anopheles messeae Fall. in the eastern part of the range // Bulletin of Tomsk State University. Biology. — 2011. — No. 2 (14). — P. 117–121.

8. Perevozkin V.P., Khalzova V.V. Species and population genetic structure of malaria mosquitoes in Central Siberia // Bulletin of Tomsk State Pedagogical University. — 2013. — No. 8 (136). — P. 75–80.

9. Moskaev A.V., Gordeev M.I. Chromosomal composition of malaria mosquitoes of the Shumikhinsky State Natural Zoological Reserve (Kurgan region) // Bulletin of Moscow State Regional University. Series: Natural Sciences. — 2015. — No. 5. — P. 26–33.

10. Gordeev M.I., Bezhonova O.V., Moskaev A.V. Chromosomal polymorphism in populations of the malaria mosquito Anopheles messeae (Diptera, Culicidae) in the south of the Russian Plain // Russian journal of Genetics. — 2012. — Vol. 48, No. 9. — P. 1124–1128.

11. Perevozkin V.P., Bondarchuk S.S., Minich A.S. Cytogenetic analysis of species composition and inversion structure of malaria mosquito populations in Astrakhan region // Russian journal of Genetics. — 2015. — Vol. 51, No. 8. — P. 924–932. https://doi.org/10.7868/S0016675815060132

12. Perevozkin V.P., Minich A.S., Bagautdinova G.T. Population and species structure of malaria mosquitoes in Central Europe // Bulletin of Tomsk State Pedagogical University. — 2013. — No. 8 (136). — P. 70–74.

13. Gordeev M.I., Sibataev A.K. Cytogenetic and phenotypic variability in central and peripheral populations of the malaria mosquito Anopheles messeae Fall. (Diptera, Culicidae) // Genetics. — 1996. — Vol. 32, No. 9. — P. 1199–1205.

14. Abylkassymova G.M., Wasserlauf I.E., Sibataev A.K., Stegniy V.N., Andreeva Yu.V., Sibataeva A.M., Pervilyeva A.V. Comparative analysis of the variability of the genetic structure of Anopheles messeae (Diptera, Culicidae) populations from Western Siberia and Eastern Kazakhstan. 2019. IOP Conf. Ser.: Earth Environ. Sci. — 400. 012013. DOI 10.1088/1755-1315/400/1/012013

15. Sibataev A.K., Shabanova Yu.V. Morfologiya malyarijnyh komarov kompleksa Anopheles maculipennis // Nauchno-prakticheskoe rukovodstvo po malyarii (epidemiologiya, sistematika, genetika) / pod red. V.N. Stegniya. — 2007. — S. 146–185.

16. Gucevich A.V., Monchadskij A.S., Shtakel'berg A.A. Komary. Semejstvo Culicidae // Fauna SSSR. Dvukrylye nasekomye. — Leningrad: Nauka, 1970. — T. 3, vyp. 4. — 384 s.

17. Kabanova V.M., Kartashova N.N., Stegniy V.N. Karyological study of natural populations of the malaria mosquito in the Middle Ob region. Communication I. Characteristics of the karyotype of Anopheles maculipennis messeae // Cytology. — 1972. — Vol. 14, No. 5. — P. 630–636.

18. Sibataev A.K., Wasserlauf I.E., Usov K.E., Alekseeva S.S., Abylkassymova G.M., Stegniy V.N. Geographic variability of inversion polymorphism of the malaria mosquito Anopheles messeae Fall. (Diptera: Culicidae) in Tomsk oblast // Russian journal of Genetics. — 2022. — Vol. 58, No. 6. — P. 678–684. DOI: 10.31857/S0016675822060091

19.