EFFECT OF DIFFERENT NUTRIENT MEDIA COMPOSITIONS ON THE MORPHOGENESIS OF POTATO VARIETIES «ROMANO» AND «RIVIERA» IN VITRO CULTURE

Potato (Solanum tuberosum L.) is one of the leading agricultural crops in the Republic of Kazakhstan. However, its productivity is declining due to viral diseases and the ineffectiveness of traditional seed production, necessitating the use of biotechnological methods to obtain improved seed material. determine the optimal conditions for mineral and hormonal nutrition to increase the efficiency of growth and development of microplants of Romano and Riviera varieties in in vitro culture. Cultivation was carried out on modified Murashige-Skoog and White media with varying sterilization, hormonal supplements, and macro- and microelement concentrations. Morphogenesis, root system development, and the multiplication rate were assessed. Variety-specific responses to the medium composition were identified; optimization of mineral and hormonal nutrition contributed to enhanced morphogenesis and an increase in the multiplication rate, which allows for the improvement of technologies for the production of improved planting material.

1. Kenshimov A. Analysis of the Agricultural Industry / Almaty. - 2019. - pp. 5-81

2. Anisimov B.V., Simakov E.A., Zhevora S.V. et al. Potatoes: Production, Consumption, Market Trends, Quality Regulations, Forecasts and Prospects. - Cheboksary: Novoye Vremya, 2022. - 66 p.

3. "World revised figures show halt in rise in potato production" / February 25, 2020. https://potatocongress.org/world-revised-figures-show-halt-in-rise-in-potato-production

4. Nutritional composition of potatoes and their impact on human health - Literature Review // Europotat - 2024, April.

5. Toqbergenova Zh.A. Kartop daqylyn microklondy kobeytu. - Almaty, 2008. 30 b.

6. Argynbayeva, AM, Daurov, DL, Sapakhova, ZB, Zhambakin, KZ, Shamekova, MK. (2023). Potato viruses in Kazakhstan and methods for obtaining virus-free seed material. Bulletin of the LN Gumilyov Eurasian National University. Bioscience Series, 144(3), 54-68. DOI: 10.32523/2616-7034-2023-144-3- 54-68.

7. Tessema, L., Kakuhenzire, R., Seid, E., Tafesse, S., Tadesse, Y., Negash, K., McEwan, M. (2024). Detection of six potato viruses using double antibody sandwich ELISA from in vitro, screen house, and field-grown potato crops in Ethiopia. Discover Applied Sciences, 6(3), 79. DOI: 10.1007/s42452-024-06832-9.

8. Gopal, J., Khurana, S.M.P. Handbook of Potato Production, Improvement, and Postharvest Management. - CRC Press, 2021. - 624 p.

9. Efficiency of applying biotechnological methods in original potato seed production at the Kemerovo Research Institute of Agriculture // Development of new breeding technologies and creation of a highquality competitive potato seed stock: Proc. of the international scientific and practical conference. - 2016. - pp. 72-76.

10. Khodaeva V. P., Kulikova V. I. Propagation of potato varieties in vitro culture on various nutrient media // Achievements of science and technology in the agro-industrial complex. - 2016. - Vol. 30, No. 10. - Pp. 66-68.

11. Romanova M.S., Khaksar E.V., Novikov O.O., Leonova N.I., Semenov A.G. The effect of different compositions of growth media on the development of microplants of the Antonina potato variety. Siberian Herald of Agricultural Science. 2020;50(6):26-36.

12. Novikov O.O., Romanova M.S., Khaksar E.V., Leonova N.I., Kosinova E.I. The effects of different compositions of growth media on the development of microplants of the Solnechny potato variety. Siberian Herald of Agricultural Science. 2021;51(6): 11-21.

13. Demchuk I. V. On the need for highly productive lines in the improvement of potato varieties by biotechnological methods // Potato growing: collection of scientific papers. Minsk, 2007. Vol. 13. pp. 27­ 37