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Microclonal propagation of grain legume crops in vitro conditions

https://doi.org/10.54596/2958-0048-2024-4-135-140

Abstract

An enhanced method of microclonal propagation of grain legume seeds, including the stages of sterilization of explants of grain legumes (peas, vetch, beans, lentils, chickpeas) their introduction into in vitro conditions, actual propagation and rooting of regenerants is presented in the article. Methods for culturing isolated plant organs, tissues and cells emerged more than half a century ago and have been constantly improved since then. Work in this direction has led to certain achievements in understanding the process of plant development.

About the Authors

A. V. Kudrin
Manash Kozybaev North Kazakhstan University NJC
Kazakhstan

Student

Petropavlovsk

 



D. Zh. Zholaushin
Manash Kozybaev North Kazakhstan University NJC
Kazakhstan

Student

Petropavlovsk



E. Ye. Kantarbayeva
Manash Kozybaev North Kazakhstan University NJC
Kazakhstan

Corresponding author, PhD, Associate Professor of the Department of Agronomy 
and Forestry

Petropavlovsk



G. A. Baiseit
Manash Kozybaev North Kazakhstan University NJC
Kazakhstan

Senior Lecturer of the Department of Agronomy and Forestry

Petropavlovsk



References

1. Sidorov V.A. Plant biotechnology. Cell selection. - Kyiv: Naukova Dumka, 1990. - P. 36-37.

2. Timofeeva O.A., Rumyantseva N.I. Plant cell and tissue culture. - Kazan: Kazan (Volga Region) Federal University, 2012. - 90 p.

3. Surkova G.N., Bobkov S.V., Soboleva G.V. Cloning technologies of legumes and cereal crops (methodological recommendations). - Moscow: Russian Agricultural Academy, 2005. - 20 p.

4. Butenko R.G. In vitro biotechnology of higher plant cells and biotechnologies based on them. - Moscow: FBK Press, 2000. - 159 p.

5. Polevoy V.V., Chirkova T.V., Lutova L.A., et al. Practical training in plant growth and stability: a textbook. - St. Petersburg: Publishing house of St. Petersburg University, 2001. - 212 p.


Review

For citations:


Kudrin A.V., Zholaushin D.Zh., Kantarbayeva E.Ye., Baiseit G.A. Microclonal propagation of grain legume crops in vitro conditions. Vestnik of M. Kozybayev North Kazakhstan University. 2024;(4 (64)):135-140. https://doi.org/10.54596/2958-0048-2024-4-135-140

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ISSN 2958-003X (Print)
ISSN 2958-0048 (Online)