ASSESSMENT OF THE EFFECT OF SUBLETHAL RADIATION DOSE ON THE INDICATORS OF LIPOPEROXIDATION IN RATS AGAINST THE BACKGROUND OF IMMOBILIZATION STRESS

The aim of the study was to study and evaluate the effect of a sublethal dose of gamma radiation (6 Gy) on the parameters of lipoperoxidation in immunocompetent organs and cells against the background of immobilization stress. The study was conducted on 40 male Wistar rats divided into 4 groups: group I-intact; group II — exposed to immobilization stress after 25 hours; group III - exposed to gamma radiation; and group IV - exposed to combined exposure (immobilization stress and gamma radiation). To reproduce emotional stress, experimental animals were kept in a special device, where the animals were motionless for 6 hours, under bright lights. They were removed from the experiment 25 hours after stress, by decapitation on the background of light ether anesthesia. Animals of groups III and IV were irradiated on the TERAGAM Сo60 radiotherapy unit ("ISOTREND spol. s.r.o.", Czech Republic) once, 6 Gy. Before irradiation was carried out topographic and dosimetry preparation of experimental animals to irradiation: an object placed on the table isocentric therapy x-ray simulator "Tigah" (Czech Republic), which designs and parameters corresponds to the therapeutic table of gamma irradiator. Ionizing radiation combined with immobilization stress has a more pronounced effect than with separate action. The combined effects of immobilization stress and ionizing radiation led to an increase in the level of DC and MDA, which lead to the development of double oxidative stress in the studied objects.

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