koz"Вестник Северо-Казахстанского университета имени Манаша Козыбаева"Bulletin of Manash Kozybayev North Kazakhstan University2958-003X2958-0048М. Қозыбаев атындағы СҚУ10.54596/2958-0048-2026-3-107-121koz-2927Research ArticleБИОЛОГИЧЕСКИЕ НАУКИBIOLOGICAL SCIENCESАНТИФУНГАЛЬНАЯ АКТИВНОСТЬ И GC-MS-ПРОФИЛЬ ГИДРОЭТАНОЛЬНОГО ЭКСТРАКТА CHELIDONIUMMAJUS L. В ОТНОШЕНИИ FUSARIUM SPP. КАРТОФЕЛЯANTIFUNGAL ACTIVITY AND GC-MS PROFILING OF A HYDROETHANOLIC EXTRACT OF CHELIDONIUM MAJUS L. AGAINST POTATO-ASSOCIATED FUSARIUM SPPhttps://orcid.org/0000-0003-4526-7115ДостемесоваА. Б.DostemesovaA. B.

Алматы

Doctoral student

Almaty

anardostemesova@gmail.comhttps://orcid.org/0009-0007-4036-3825КамароваД. Н.KamarovaD. N.

Астана

corresponding author, doctoral student, master of Agricultural Sciences

Astana

dkamarova10@gmail.comhttps://orcid.org/0000-0002-9194-2727ӘжімаханМ. Ә.AzhimakhanM. A.

Астана

PhD, lecturer

Astana

moldirkazatu@mail.ruhttps://orcid.org/0000-0002-7670-5352СүлейманМ. А.SuleimanM. A.

Астана

Master of Agricultural Sciences, lecturer

Astana

suleimanmadina@mail.ruhttps://orcid.org/0000-0002-9054-5551ХасановВ. Т.KhassanovV. T.

Астана

Candidate of Biological Sciences, professor,

Astana

vadimkazgatu@mail.ruhttps://orcid.org/0000-0002-6418-1950ИзбастинаК. С.IzbastinaK. S.

Астана

corresponding author, PhD, associate professor

Astana

izbastina.k@gmail.comhttps://orcid.org/0000-0001-5921-3113МухтубаеваС. К.MukhtubaevaS. K.

Астана

Candidate of biological Sciences, associate Professor

Astana

mukhtubaeva@mail.ruhttps://orcid.org/0000-0002-9467-1483ШариповаМ. Ж.SharipovaM. Z.

Астана

PhD, associate professor Astana International University

Astana

moldirzhumagul@gmail.comКазахский национальный университет имени аль-ФарабиКазахстанAl-Farabi Kazakh National UniversityKazakhstanНАО «Казахский агротехнический исследовательский университет им. С. Сейфуллина»КазахстанNCJSC «S. Seiffulin Kazakh Agro Technical University»KazakhstanНАО «Казахский агротехнический исследовательский университет им. С. Сейфуллина»; «Астанинский ботанический сад» филиал РГП на ПХВ «Институт ботаники и фитоинтродукции» Комитета лесного хозяйства и животного мира Министерства экологии и природных ресурсов Республики КазахстанКазахстанNCJSC «S. Seiffulin Kazakh Agro Technical University»; RSE on the REM “Astana Botanical Garden ” Committee of Forestry and Animal World of the Ministry of Ecology and Nature ConservationKazakhstan«Астанинский ботанический сад» филиал РГП на ПХВ «Институт ботаники и фитоинтродукции» Комитета лесного хозяйства и животного мира Министерства экологии и природных ресурсов Республики Казахстан; Международный университет АстанаКазахстанRSE on the REM “Astana Botanical Garden ” Committee of Forestry and Animal World of the Ministry of Ecology and Nature Conservation; Astana International UniversityKazakhstanМеждународный университет АстанаКазахстанAstana International UniversityKazakhstan20263009202603 (71)107121Copyright © Достемесова А.Б., Камарова Д.Н., Әжімахан М.Ә., Сүлейман М.А., Хасанов В.Т., Избастина К.С., Мухтубаева С.К., Шарипова М.Ж., 20262026Достемесова А.Б., Камарова Д.Н., Әжімахан М.Ә., Сүлейман М.А., Хасанов В.Т., Избастина К.С., Мухтубаева С.К., Шарипова М.Ж.Dostemesova A.B., Kamarova D.N., Azhimakhan M.A., Suleiman M.A., Khassanov V.T., Izbastina K.S., Mukhtubaeva S.K., Sharipova M.Z.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik.ku.edu.kz/jour/article/view/2927

В статье представлен предварительный GC-MS-профиль и антифунгальная активность гидроэтанольного экстракта Chelidonium majus L., собранного в высокогорных условиях хребта Кунгей Алатау (Казахстан). Антифунгальную активность оценивали in vitro в отношении одного изолята Fusarium spp., выделенного из клубней картофеля с симптомами фузариозного поражения. Экстракты получали методом Сокслета с использованием 70 % этанола. Площадь мицелиального роста определяли по цифровым изображениям с использованием программы ImageJ. Эксперимент проводили в четырех биологических повторностях (n = 4). В варианте с гидроэтанольным экстрактом C. majus №1 площадь мицелиального роста Fusarium spp. составила 60,25 ± 4,08 %, тогда как в контроле растворителя с 70 % этанолом – 72,50 ± 2,65 %. Ингибирование мицелиального роста относительно контроля растворителя составило 16,9 %. Методом GC-MS в составе экстракта предположительно идентифицировано 11 соединений, среди которых значительную долю составляли этиловые эфиры ненасыщенных жирных кислот (43,4 %), фитол (13,8 %) и соединение 4H-Bis[1,3]benzodioxolo[5,6-a:4’,5’-g]quinolizine, 6,7,12b,13-tetrahydro-, (±) – (30,2 %), предположительно идентифицированное по библиотечному совпадению масс-спектров. Полученные результаты свидетельствуют об ингибирующей активности гидроэтанольного экстракта C. majus в отношении мицелиального роста Fusarium spp. in vitro и обосновывают необходимость дальнейших исследований.

 

The article presents a preliminary GC-MS profile and antifungal activity of a hydroethanol extract of Chelidonium majus L. collected in the high-altitude conditions of the Kungei Alatau range (Kazakhstan). Antifungal activity was evaluated in vitro against one Fusarium spp. isolated from potato tubers with symptoms of fusarium lesion.

The extracts were obtained by Soxhlet extraction using 70% ethanol. The mycelial growth area was determined from digital images using ImageJ software. The experiment was carried out in four repetitions (n = 4). In the treatment with hydroethanolic extract of C. majus No. 1, the mycelial growth area of Fusarium spp. was 60.25 ± 4.08%, compared with 72.50 ± 2.65% in the solvent control containing 70% ethanol. Inhibition of mycelial growth relative to the solvent control was 16.9%. GC-MS analysis tentatively identified 11 compounds in the extract, among which ethyl esters of unsaturated fatty acids accounted for a substantial proportion (43.4%), followed by phytol (13.8%) and the compound 4H-Bis[1,3]benzodioxolo[5,6-a:4’,5’-g]quinolizine, 6,7,12b,13-tetrahydro-, (±) - (30.2%), tentatively identified based on mass-spectral library matching. The results obtained indicate the inhibitory activity of the hydroethanol extract of C. majus against the mycelial growth of Fusarium spp. in vitro and justify the need for further research.

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The authors declare that there are no conflicts of interest present.