koz"Вестник Северо-Казахстанского университета имени Манаша Козыбаева"Bulletin of Manash Kozybayev North Kazakhstan University2958-003X2958-0048М. Қозыбаев атындағы СҚУ10.54596/2958-0048-2026-2-109-124koz-2779Research ArticleБИОЛОГИЧЕСКИЕ НАУКИBIOLOGICAL SCIENCESФИТОХИМИЧЕСКИЙ ПРОФИЛЬ ЭТАНОЛЬНЫХ ЭКСТРАКТОВ ЛИСТЬЕВ ЭНДЕМИЧНЫХ ВИДОВ ЗАИЛИЙСКОГО АЛАТАУ (CRATAEGUS DSUNGARICA, ROSA WEBBIANA, BERBERIS HETEROPODA) И ИХ ГЕРОПРОТЕКТОРНЫЙ ПОТЕНЦИАЛ ПО ДАННЫМ ГХ-МС АНАЛИЗАPHYTOCHEMICAL PROFILE OF ETHANOLIC LEAF EXTRACTS OF ENDEMIC TRANS-ILI ALATAU SPECIES (CRATAEGUS DSUNGARICA, ROSA WEBBIANA, BERBERIS HETEROPODA) AND THEIR HEPATOPROTECTIVE POTENTIAL AS DETERMINED BY GC-MS ANALYSIShttps://orcid.org/0000-0002-1622-3626КыдырбаеваА. К.KydyrbayevaA. K.

PhD doctoral student

Алматы

PhD doctoral student

Almaty

kydyrbaeva.a@kaznmu.kzhttps://orcid.org/0000-0001-6724-9810ШалахметоваТ. М.ShalakhmetovaT. M.

Doctor of Biological Sciences, Professor of the Department of Zoology, Histology and Cytology

Алматы

Doctor of Biological Sciences, Professor of the Department of Zoology, Histology and Cytology

Almaty

t_shalakhmetova@mail.ruhttps://orcid.org/0000-0002-4203-9419АйкешевБ. М.AikeshevB. M.

Candidate of Biological Sciences, PhD, Higher School of Natural Sciences

Астана

candidate of Biological Sciences, PhD, Higher School of Natural Sciences

Astana 

aikeshev@gmail.comКазахский национальный университет имени аль-ФарабиКазахстанAl-Farabi Kazakh National UniversityKazakhstanМеждународный университет АстанаКазахстанAstana International UniversityKazakhstan20260907202602 (70)109124Copyright © Кыдырбаева А.К., Шалахметова Т.М., Айкешев Б.М., 20262026Кыдырбаева А.К., Шалахметова Т.М., Айкешев Б.М.Kydyrbayeva A.K., Shalakhmetova T.M., Aikeshev B.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik.ku.edu.kz/jour/article/view/2779

Лекарственные растения рассматриваются как перспективные источники природных геропротекторных агентов благодаря способности снижать окислительный стресс, подавлять хроническое воспаление и замедлять развитие возраст-ассоциированных патологий. В настоящем исследовании был проведен сравнительный анализ соединений, выделенных из этанольных экстрактов листьев трех эндемичных видов флоры Зайлийского Алатау – Crataegus dsungarica Zabel. ex Lange., Rosa webbiana Wall. ex Royle и Berberis heteropoda Schrenk. ex Fisch. & C.A.Mey. с использованием газовой хроматографии-масс-спектрометрии (ГХ-МС). Разделение компонентов осуществлялось на капиллярной колонке DB-WaxEtr с использованием гелия в качестве газа-носителя, идентификация соединений проводилась на основе спектральных библиотек Wiley 7-го издания и NIST’02. Среди идентифицированных соединений доминировали сесквитерпеноид азулен-4,7-диол (27,42 %) и фитол (5,77 %), известные своими антиоксидантными и противовоспалительными свойствами. В листьях Crataegus dsungarica обнаружены терпеновые спирты - неофитадиен и фитол, а также высшие алканы. В экстракте листьев Berberis heteropoda преобладает фитол (44,58%), дополненный неофитадиеном (7,47%) и 1,2,3,5-циклогексантетролом (16,55%). Совокупность выявленных соединений - терпеноидов, фенольных соединений, ненасыщенных жирных кислот и их производных - свидетельствует о перспективности их геропротекторного действия; для его верификации необходимы дальнейшие экспериментальные исследования, направленные на изучение антиоксидантных эффектов, митохондриальной защиты и регуляции липидного метаболизма.

Medicinal plants are considered promising sources of natural geroprotective agents due to their ability to reduce oxidative stress, suppress chronic inflammation, and slow the progression of age-related pathologies. In this study, a comparative analysis was conducted of compounds isolated from ethanol extracts of the leaves of three endemic species of the Alatau flora - Crataegus dsungarica Zabel. ex Lange., Rosa webbiana Wall. ex Royle, and Berberis heteropoda Schrenk. ex Fisch. & C.A. Mey. using gas chromatography-mass spectrometry (GC-MS). The separation of components was performed on a DB-WaxEtr capillary column using helium as the carrier gas; the identification of compounds was based on the Wiley 7th edition and NIST’02 spectral libraries. Among the identified compounds, the sesquiterpenoids azulene-4,7-diol (27.42%) and phytol (5.77%), known for their antioxidant and anti-inflammatory properties, were predominant. Terpene alcohols - neophytadiene and phytol - as well as higher alkanes were detected in the leaves of Crataegus dsungarica. The leaf extract of Berberis heteropoda is dominated by phytol (44.58%), supplemented by neophytadiene (7.47%) and 1,2,3,5-cyclohexantetrol (16.55%). The combination of identified compounds-terpenoids, phenolic compounds, unsaturated fatty acids, and their derivatives - suggests that they have promising geroprotective effects; Further experimental studies are needed to verify this, focusing on the antioxidant effects, mitochondrial protection, and regulation of lipid metabolism.

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The authors declare that there are no conflicts of interest present.