koz"Вестник Северо-Казахстанского университета имени Манаша Козыбаева"Bulletin of Manash Kozybayev North Kazakhstan University2958-003X2958-0048М. Қозыбаев атындағы СҚУ10.54596/2958-0048-2026-1-79-93koz-2435Research ArticleБИОЛОГИЧЕСКИЕ НАУКИBIOLOGICAL SCIENCESБИОТЕХНОЛОГИЧЕСКИЕ МЕТОДЫ ОЗДОРОВЛЕНИЯ ПОДВОЕВ ЯБЛОНИ И ПОЛУЧЕНИЯ БЕЗВИРУСНОГО ПОСАДОЧНОГО МАТЕРИАЛА В КАЗАХСТАНЕBIOTECHNOLOGICAL METHODS FOR THE SANITATION OF APPLE ROOTSTOCKS AND THE PRODUCTION OF VIRUS-FREE PLANTINGhttps://orcid.org/0000-0002-6536-7263АсқароваМ. А.AskarovaM. A.

Алматы

hD student, Department of Biotechnology; Senior Researcher, Department of Plant Protection

Almaty

moldyraskarova1@gmail.comЮсуповаЗ. Я.YussupovaZ. Ya.

Алматы

Master of Agricultural Sciences, Researcher, "In Vitro Gene Pool" Laboratory

Almaty

zarina19851005@mail.ruНурсеитоваТ. Н.NurseitovaT. N.

Алматы

Sciences, Researcher, "In Vitro Gene Pool" Laboratory

Almaty

n.toigul90@mail.ruАбдикаримоваР. А.AbdikarimovaR. A.

Алматы

Master of Agricultural Sciences, Junior Researcher, "In Vitro Gene Pool" Laboratory

Almaty

Raigul95@mail.ruСаршаеваМ. Ж.SarshayevaM. Zh.

Алматы

Master of Natural Sciences, researcher

Almaty

moka-1993@mail.ruКабылбековаБ. Ж.KabylbekovaB. Zh.

Алматы

Head of the "In Vitro Gene Pool" Laboratory

Almaty

k_b_zh@mail.ruАО «Алматинский технологический университет»; ТОО «Казахский научно-исследовательский институт плодовощеводства»КазахстанJSC "Almaty Technological University"; LLP Kazakh Fruit and Vegetable Research InstituteKazakhstanТОО «Казахский научно-исследовательский институт плодовощеводства»КазахстанLLP Kazakh Fruit and Vegetable Research InstituteKazakhstan20260804202601 (69)7993Copyright © Асқарова М.А., Юсупова З.Я., Нурсеитова Т.Н., Абдикаримова Р.А., Саршаева М.Ж., Кабылбекова Б.Ж., 20262026Асқарова М.А., Юсупова З.Я., Нурсеитова Т.Н., Абдикаримова Р.А., Саршаева М.Ж., Кабылбекова Б.Ж.Askarova M.A., Yussupova Z.Y., Nurseitova T.N., Abdikarimova R.A., Sarshayeva M.Z., Kabylbekova B.Z.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik.ku.edu.kz/jour/article/view/2435

В промышленных садах и питомниках распространение вирусных инфекций яблони (Malus domestica Borkh.) представляет серьёзную угрозу фитосанитарному состоянию и продуктивности растений. Целью исследования было получение безвирусного посадочного материала подвоев яблони и оценка эффективности комплексных биотехнологических методов оздоровления in vitro. Объектом исследования были подвои яблони из помологических и in vitro коллекций Казахстана. Наибольшая вирусная нагрузка была выявлена в подвое Б-16-20. Для оздоровления применяли комбинированный метод Thermotherapy + Chemotherapy + SAM с использованием противовирусных препаратов. Результаты показали, что эффективность и фитотоксичность методов зависят от сочетания методов и концентрации препаратов. Полученные данные подтверждают высокую эффективность комплексного применения биотехнологических подходов для получения безвирусного посадочного материала яблони и имеют практическое значение для сертифицированного размножения плодовых культур в Казахстане.

In commercial orchards and nurseries, the spread of viral infections in apple (Malus domestica Borkh.) poses a serious threat to plant phytosanitary status and productivity. The aim of this study was to obtain virus-free apple rootstock planting material and to evaluate the effectiveness of integrated in vitro biotechnological methods of plant sanitation. The objects of the study were apple rootstocks from pomological and in vitro collections in Kazakhstan. The highest viral load was detected in rootstock B-16-20. For sanitation, a combined Thermotherapy + Chemotherapy + SAM method with antiviral agents was applied. The results showed that the effectiveness and phytotoxicity of the methods depend on the combination of techniques and the concentration of the applied agents. The obtained data confirm the high efficiency of integrated biotechnological approaches for obtaining virus-free apple planting material and have practical significance for certified propagation of fruit crops in Kazakhstan.

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The authors declare that there are no conflicts of interest present.