koz"Вестник Северо-Казахстанского университета имени Манаша Козыбаева"Bulletin of Manash Kozybayev North Kazakhstan University2958-003X2958-0048М. Қозыбаев атындағы СҚУkoz-148Research ArticleЕСТЕСТВЕННЫЕ НАУКИNATURAL SCIENCESВЫРАЖЕННОСТЬ СИГМЫ – 1 РЕЦЕПТОРОВ СПИНАЛЬНЫХ ГАНГЛИЙ В КОРЕШКЕ СПИННОГО МОЗГА: ПОВТОРНАЯ ЭКСПЕРТИЗА С ИСПОЛЬЗОВАНИЕМ ВЕСЬМА СПЕЦИФИЧЕСКИХ АНТИТЕЛSIGMA – 1 RECEPTOR EXPRESSION IN THE DORSAL ROOT GANGLION: REEXAMINATION USING A HIGHLY SPECIFIC ANTIBODYMaвлютовТ. А.MavlyutovT. A.

Мэдисон

Madison

ДюлльманТ.DuellmanT.

Мэдисон

Madison

KимТэ ХенKimHung Tae

Мэдисон

Madison

ЭпштейнМ. Л.EpsteinM. L.

Мэдисон

Madison

ЛизШ.LeeseCh.

Южный Йоркшир, Англия

England

Университет Висконсина, Школа медицины и общественного здравохраненияСоединённые Штаты АмерикиUniversity of Wisconsin, School of Medicine and Public HealthUnited StatesШеффилдский университетВеликобританияUniversity of Sheffield, South YorkshireUnited Kingdom20181108202103 (40)621Copyright © Maвлютов Т.А., Дюлльман Т., Kим Т., Эпштейн М.Л., Лиз Ш., 20212021Maвлютов Т.А., Дюлльман Т., Kим Т., Эпштейн М.Л., Лиз Ш.Mavlyutov T.A., Duellman T., Kim H., Epstein M.L., Leese C.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik.ku.edu.kz/jour/article/view/148

Бұл жұмыста қондырғы ПФ режимінде жұмыс істеген кезде плазма параметрлерін есептеу және коаксиалды цилиндрлік электродтарды плазмалық фокус геометриясына ауыстыру бойынша эксперимент қою міндеті қойылады. Фокустың іргелі параметрлері есептелген, плазмалық фокустың қалыптасу шарттарын алдын ала бағалау нәтижелері көрсетілген. Электродинамикалық модель формулалары бойынша электрондардың температурасы мен концентрациясының максималды мәні анықталды. Электродинамикалық модель бойынша есептеулер электрондар концентрациясының анод радиусынан тәуелділігін көрсетеді. Сондай– ақ, электронды температураның өсуіне ток күшінің артуы әсер етеді, бірақ импульс уақыты азаяды. Анод радиусы 0,25 см–ден 2,25 см–ге дейін өзгерген кезде электрондық температураның мәнін анықтауға мүмкіндік беретін деректер алынды. Разряд тогының кернеу шамасына тәуелділігі көрсетілген. Жүргізілген эксперименттер, егер жүйенің индуктивтілігін айтарлықтай төмендету бойынша шаралар қолданбаса, плазманың динамикалық параметрлері пинчты қатты қысу үшін жеткіліксіз екенін көрсетті.Сигма – 1 рецептор (S1R) – уникальный плюрипотентный модулятор живых систем, связанный со многими неврологическими состояниями, в том числе с патологическими болями. Антагонисты Сигма – 1 рецепторов уменьшают интенсивность неврологической боли у грызунов. Локализация S1R в спинном мозге показывает распределение мотонейронов, предполагая высокую вероятность скопления S1R в спинном нервном узле, что позволяет снизить интенсивность боли путем применения определенных лекарственных средств. Мы исследовали нервные спинные узлы мышей и крыс на присутствие Сигма – 1 рецепторов и выяснили, что, судя по уровню белка и результатам ПЦР теста, в спинных нервных узлах содержится больше Сигма – 1 рецепторов по сравнению со спинным мозгом, корой головного мозга и легкими, но меньше, чем в печени. Используя заранее изготовленные антитела, мы продемонстрировали присутствие сильной иммунофлюоресценции Сигма – 1 рецепторов в спинных ганглиях у крыс и мышей, co – локализацию нейронов спинных нервных узлов с маркером NSE, за исключением GFAP – положительных глиальн ых спутниковых клеток. Кроме того, S1R отсутствовали в центростремительных терминалах в коже и в переднем роге спинного мозга. Используя иммунно – электронную микроскопию, мы показали, что S1R обнаружены в ядерной мембране и эндоплазматической сети клеток спинных нервных узлов. В отличие от других клеток, S1R также расположен непосредственно в плазменной мембране нейронов спинных ганглий. Присутствие S1R в ядерной мембране всех нейронов спинных нервных узлов предполагает захватывающую потенциальную роль S1R как регулятора нейронных процессов и экспрессии генов, что позволит регулировать физиологическую боль путем целенаправленного воздействия на афферентные нейроны. 2016 IBRO. Изданный Elsevier Ltd. Все права защищены.

Sigma – 1 receptor (S1R) is a unique pluripotent modulator of living systems and has been reported to be associated with a number of neurological diseases including pathological pain. Intrathecal administration of S1R antagonists attenuates the pain behavior of rodents in both inflammatory and neuropathic pain models. However, the S1R localization in the spinal cord shows a selective ventral horn motor neuron distribution, suggesting the high likelihood of S1R in the dorsal root ganglion (DRG) mediating the pain relief by intrathecally administered drugs. Since primary afferents are the major component in the pain pathway, we examined the mouse and rat DRGs for the presence of the S1R. At both mRNA and protein levels, quantitative RT – PCR (qRT – PCR) and Western confirmed that the DRG contains greater S1R expression in comparison to spinal cord, cortex, or lung but less than liver. Using a custom – made highly specific antibody, we demonstrated the presence of a strong S1R immuno – fluorescence in all rat and mouse DRG neurons co – localizing with the Neuron – Specific Enolase (NSE) marker, but not in neural processes or GFAP – positive glial satellite cells. In addition, S1R was absent in afferent terminals in the skin and in the dorsal horn of the spinal cord. Using immuno – electron microscopy, we showed that S1R is detected in the nuclear envelope and endoplasmic reticulum (ER) of DRG cells. In contrast to other cells, S1R is also located directly at the plasma membrane of the DRG neurons. The presence of S1R in the nuclear envelope of all DRG neurons suggests an exciting potential role of S1R as a regulator of neuronal nuclear activities and / or gene expression, which may provide insight toward new molecular targets for modulating nociception at the level of primary afferent neurons. 2016 IBRO. Published by Elsevier Ltd. All rights reserved.

Сигма 1 рецептор (S1R)спинной нервный узел (DRG)больSigma – 1 receptorS1Rdorsal root ganglionDRGpainReferencesAlonso G, Phan V, Guillemain I, Saunier M, Legrand A, Anoal M, Maurice T (2000) Immunocytochemical localization of the sigma1 receptor in the adult rat central nervous system. Neuroscience 97:155 – 170.Alonso G, Phan V, Guillemain I, Saunier M, Legrand A, Anoal M, Maurice T (2000) Immunocytochemical localization of the sigma1 receptor in the adult rat central nervous system. Neuroscience 97:155 – 170.Bangaru ML, Weihrauch D, Tang QB, Zoga V, Hogan Q, Wu H (2013) Sigma – 1 receptor expression in sensory neurons and the effect of painful peripheral nerve injury. Mol Pain 9:47.Bangaru ML, Weihrauch D, Tang QB, Zoga V, Hogan Q, Wu H (2013) Sigma – 1 receptor expression in sensory neurons and the effect of painful peripheral nerve injury. Mol Pain 9:47.Cenda´ n CM, Pujalte JM, Portillo – Salido E, Montoliu L, Baeyens JM (2005) Formalin – induced pain is reduced in sigma 1 receptor knockout mice. Eur J Pharmacol 511:73 – 74.Cenda´ n CM, Pujalte JM, Portillo – Salido E, Montoliu L, Baeyens JM (2005) Formalin – induced pain is reduced in sigma 1 receptor knockout mice. Eur J Pharmacol 511:73 – 74.Costa FAL, Neto FLM (2015) Satellite glial cells in sensory ganglia: its role in pain. Braz J Anesthesiol English Ed 65:73 – 81.Costa FAL, Neto FLM (2015) Satellite glial cells in sensory ganglia: its role in pain. Braz J Anesthesiol English Ed 65:73 – 81.Darios F, Niranjan D, Ferrari E, Zhang F, Soloviev M, Rummel A, Bigalke H, Suckling J, Ushkaryov Y, Naumenko N, Shakirzyanova A, Giniatullin R, Maywood E, Hastings M, Binz T, DavletovB (2010) SNARE tagging allows stepwise assembly of a multimodular medicinal toxin. Proc Natl Acad Sci U S A 107:18197 – 18201.Darios F, Niranjan D, Ferrari E, Zhang F, Soloviev M, Rummel A, Bigalke H, Suckling J, Ushkaryov Y, Naumenko N, Shakirzyanova A, Giniatullin R, Maywood E, Hastings M, Binz T, DavletovB (2010) SNARE tagging allows stepwise assembly of a multimodular medicinal toxin. Proc Natl Acad Sci U S A 107:18197 – 18201.Davis MP (2015) Sigma – 1 receptors and animal studies centered on pain and analgesia. Expert Opin Drug Discov 0441:1 – 16. Dittmer A, Dittmer J (2006)b – Actin is not a reliable loading control in Western blot analysis. Electrophoresis 27:2844 – 2845.Davis MP (2015) Sigma – 1 receptors and animal studies centered on pain and analgesia. Expert Opin Drug Discov 0441:1 – 16. Dittmer A, Dittmer J (2006)b – Actin is not a reliable loading control in Western blot analysis. Electrophoresis 27:2844 – 2845.Entrena JM, Cobos EJ, Nieto FR, Cenda´ n CM, Gris G, Del Pozo E, Zamanillo D, Baeyens JM (2009) Sigma – 1 receptors are essential for capsaicin – induced mechanical hypersensitivity: studies with selective sigma – 1 ligands and sigma – 1 knockout mice. Pain 143:252 – 261.Entrena JM, Cobos EJ, Nieto FR, Cenda´ n CM, Gris G, Del Pozo E, Zamanillo D, Baeyens JM (2009) Sigma – 1 receptors are essential for capsaicin – induced mechanical hypersensitivity: studies with selective sigma – 1 ligands and sigma – 1 knockout mice. Pain 143:252 – 261.Ferguson RE, Carroll HP, Harris A, Maher ER, Selby PJ, Banks RE, Banks R (2005) Housekeeping proteins: a preliminary study illustrating some limitations as useful references in protein expression studies. Proteomics 5:566 – 571.Ferguson RE, Carroll HP, Harris A, Maher ER, Selby PJ, Banks RE, Banks R (2005) Housekeeping proteins: a preliminary study illustrating some limitations as useful references in protein expression studies. Proteomics 5:566 – 571.Ferrari E, Gu C, Niranjan D, Restani L, Rasetti – Escargueil C, Obara I, Geranton SM, Arsenault J, Goetze TA, Harper CB, Nguyen TH (2013) Synthetic self – assembling clostridial chimera for modulation of sensory functions. Bioconjugate Chem 24:1750 – 1759.Ferrari E, Gu C, Niranjan D, Restani L, Rasetti – Escargueil C, Obara I, Geranton SM, Arsenault J, Goetze TA, Harper CB, Nguyen TH (2013) Synthetic self – assembling clostridial chimera for modulation of sensory functions. Bioconjugate Chem 24:1750 – 1759.Fontanilla D, Johannessen M, Hajipour AR, Cozzi NV, Jackson MB, Ruoho AE (2009) The hallucinogen N,N – dimethyltryptamine (DMT) is an endogenous sigma – 1 receptor regulator. Science 323:934 – 937.Fontanilla D, Johannessen M, Hajipour AR, Cozzi NV, Jackson MB, Ruoho AE (2009) The hallucinogen N,N – dimethyltryptamine (DMT) is an endogenous sigma – 1 receptor regulator. Science 323:934 – 937.Gris G, Cobos EJ, Zamanillo D, Portillo – Salido E (2015) Sigma – 1 receptor and inflammatory pain. Inflamm Res:377 – 381.Gris G, Cobos EJ, Zamanillo D, Portillo – Salido E (2015) Sigma – 1 receptor and inflammatory pain. Inflamm Res:377 – 381.Hanani M (2005) Satellite glial cells in sensory ganglia: from form to function. Brain Res Rev 48:457 – 476.Hanani M (2005) Satellite glial cells in sensory ganglia: from form to function. Brain Res Rev 48:457 – 476.Hartl FU, Bracher A, Hayer – Hartl M (2011) Molecular chaperones in protein folding and proteostasis. Nature 475:324 – 332.Hartl FU, Bracher A, Hayer – Hartl M (2011) Molecular chaperones in protein folding and proteostasis. Nature 475:324 – 332.Hayashi T, Su TP (2007) Sigma – 1 receptor chaperones at the ERmitochondrion interface regulate Ca2+signaling and cell survival. Cell 131:596 – 610.Hayashi T, Su TP (2007) Sigma – 1 receptor chaperones at the ERmitochondrion interface regulate Ca2+signaling and cell survival. Cell 131:596 – 610.Kim HW, Kwon YB, Roh DH, Yoon SY, Han HJ, Kim KW, Beitz AJ, Lee JH (2006) Intrathecal treatment with sigma1 receptor antagonists reduces formalin – induced phosphorylation of NMDA receptor subunit 1 and the second phase of formalin test in mice. Br J Pharmacol 148:490 – 498.Kim HW, Kwon YB, Roh DH, Yoon SY, Han HJ, Kim KW, Beitz AJ, Lee JH (2006) Intrathecal treatment with sigma1 receptor antagonists reduces formalin – induced phosphorylation of NMDA receptor subunit 1 and the second phase of formalin test in mice. Br J Pharmacol 148:490 – 498.Kourrich S, Hayashi T, Chuang JY, Tsai SY, Su TP, Bonci A (2013) Dynamic interaction between sigma – 1 receptor and Kv1.2 shapes neuronal and behavioral responses to cocaine. Cell 152:236 – 247.Kourrich S, Hayashi T, Chuang JY, Tsai SY, Su TP, Bonci A (2013) Dynamic interaction between sigma – 1 receptor and Kv1.2 shapes neuronal and behavioral responses to cocaine. Cell 152:236 – 247.Kuchipudi SV, Tellabati M, Nelli RK, White GA, Perez BB, Sebastian S, Slomka MJ, Brookes SM, Brown IH, Dunham SP, Chang KC (2012) 18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells. Virol J 9:230.Kuchipudi SV, Tellabati M, Nelli RK, White GA, Perez BB, Sebastian S, Slomka MJ, Brookes SM, Brown IH, Dunham SP, Chang KC (2012) 18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells. Virol J 9:230.Martin WR, Eades CG, Thompson JA, Huppler RE, Gilbert PE (1976) The effects of morphine and nalorphine – like drugs in the nondependent and morphine – dependent chronic spinal dog.J Pharmacol Exp Ther 197:517 – 532.Martin WR, Eades CG, Thompson JA, Huppler RE, Gilbert PE (1976) The effects of morphine and nalorphine – like drugs in the nondependent and morphine – dependent chronic spinal dog.J Pharmacol Exp Ther 197:517 – 532.Mavlyutov TA, Epstein ML, Andersen KA, Ziskind – Conhaim L, Ruoho AE (2010) The sigma – 1 receptor is enriched in postsynaptic sites of C – terminals in mouse motoneurons. An anatomical and behavioral study. Neuroscience 167:247 – 255.Mavlyutov TA, Epstein ML, Andersen KA, Ziskind – Conhaim L, Ruoho AE (2010) The sigma – 1 receptor is enriched in postsynaptic sites of C – terminals in mouse motoneurons. An anatomical and behavioral study. Neuroscience 167:247 – 255.Mavlyutov TA, Guo LW, Epstein ML, Ruoho AE (2015a) Role of the Sigma – 1 receptor in amyotrophic lateral sclerosis (ALS). J Pharmacol Sci 127:10 – 1 6.Mavlyutov TA, Guo LW, Epstein ML, Ruoho AE (2015a) Role of the Sigma – 1 receptor in amyotrophic lateral sclerosis (ALS). J Pharmacol Sci 127:10 – 1 6.Mavlyutov TA, Epstein M, Guo LW (2015b) Subcellular localization of the sigma – 1 receptor in retinal neurons – an electron microscopy study. Sci Rep 5:1 – 11.Mavlyutov TA, Epstein M, Guo LW (2015b) Subcellular localization of the sigma – 1 receptor in retinal neurons – an electron microscopy study. Sci Rep 5:1 – 11.Mitsuda T, Omi T, Tanimukai H, Sakagami Y, Tagami S, Okochi M, Kudo T, Takeda M (2011) Sigma – 1Rs are upregulated via PERK/ eIF2ahpha/ATF4 pathway and execute protective function in ER stress. Biochem Biophys Res Commun 415:519 – 525.Mitsuda T, Omi T, Tanimukai H, Sakagami Y, Tagami S, Okochi M, Kudo T, Takeda M (2011) Sigma – 1Rs are upregulated via PERK/ eIF2ahpha/ATF4 pathway and execute protective function in ER stress. Biochem Biophys Res Commun 415:519 – 525.Moebius FF, Reiter RJ, Hanner M, Glossmann H (1997) High affinity of sigma 1 – binding sites for sterol isomerization inhibitors: evidence for a pharmacological relationship with the yeast sterol C8 – C7 isomerase. Br J Pharmacol 121:1 – 6.Moebius FF, Reiter RJ, Hanner M, Glossmann H (1997) High affinity of sigma 1 – binding sites for sterol isomerization inhibitors: evidence for a pharmacological relationship with the yeast sterol C8 – C7 isomerase. Br J Pharmacol 121:1 – 6.Omi T, Tanimukai H, Kanayama D, Sakagami Y, Tagami S, Okochi M, Morihara T, Sato M, Yanagida K, Kitasyoji A, Hara H, Imaizumi K, Maurice T, Chevallier N, Marchal S, Takeda M, Kudo T (2014) Fluvoxamine alleviates ER stress via induction of Sigma – 1 receptor. Cell Death Dis 5:e1332.Omi T, Tanimukai H, Kanayama D, Sakagami Y, Tagami S, Okochi M, Morihara T, Sato M, Yanagida K, Kitasyoji A, Hara H, Imaizumi K, Maurice T, Chevallier N, Marchal S, Takeda M, Kudo T (2014) Fluvoxamine alleviates ER stress via induction of Sigma – 1 receptor. Cell Death Dis 5:e1332.Prasad PD, Li HW, Fei YJ, Ganapathy ME, Fujita T, Plumley LH, Yang – Feng TL, Leibach FH, Ganapathy V (1998) Exon – intron structure, analysis of promoter region, and chromosomal localization of the human type 1 sigma receptor gene. J Neurochem 70:443 – 451.Prasad PD, Li HW, Fei YJ, Ganapathy ME, Fujita T, Plumley LH, Yang – Feng TL, Leibach FH, Ganapathy V (1998) Exon – intron structure, analysis of promoter region, and chromosomal localization of the human type 1 sigma receptor gene. J Neurochem 70:443 – 451.Ramachandran S, Lu H, Prabhu U, Ruoho AE (2007) Purification and characterization of the guinea pig sigma – 1 receptor functionally expressed inEscherichia coli. Protein Expr Purif 51:283 – 292.Ramachandran S, Lu H, Prabhu U, Ruoho AE (2007) Purification and characterization of the guinea pig sigma – 1 receptor functionally expressed inEscherichia coli. Protein Expr Purif 51:283 – 292.Ramachandran S, Chu UB, Mavlyutov TA, Pal A, Pyne S, Ruoho AE (2009)The sigma1 receptor interacts with N – alkyl amines and endogenous sphingolipids. Eur J Pharmacol 609:19 – 26.Ramachandran S, Chu UB, Mavlyutov TA, Pal A, Pyne S, Ruoho AE (2009)The sigma1 receptor interacts with N – alkyl amines and endogenous sphingolipids. Eur J Pharmacol 609:19 – 26.Sapunar D, Kostic S, Banozic A, Puljak L (2012) Dorsal root ganglion – a potential new therapeutic target for neuropathic pain. J Pain Res 5:31 – 38.Sapunar D, Kostic S, Banozic A, Puljak L (2012) Dorsal root ganglion – a potential new therapeutic target for neuropathic pain. J Pain Res 5:31 – 38.Schmidt HR, Zheng S, Gurpinar E, Koehl A, Manglik A, Kruse AC (2016) Crystal structure of the human S1 receptor. Nature 532:527 – 530.Schmidt HR, Zheng S, Gurpinar E, Koehl A, Manglik A, Kruse AC (2016) Crystal structure of the human S1 receptor. Nature 532:527 – 530.Seth P, Leibach FH, Ganapathy V (1997) Cloning and structural analysis of the cDNA and the gene encoding the murine type1 sigma receptor. Biochem Biophys Res Commun 241:535 – 540.Seth P, Leibach FH, Ganapathy V (1997) Cloning and structural analysis of the cDNA and the gene encoding the murine type1 sigma receptor. Biochem Biophys Res Commun 241:535 – 540.Storek B, Reinhardt M, Wang C, Janssen WGM, Harder NM, Banck MS, Morrison JH, Beutler AS (2008) Sensory neuron targeting by self – complementary AAV8 via lumbar puncture for chronic pain. Proc Natl Acad Sci U S A 105:1055 – 1060.Storek B, Reinhardt M, Wang C, Janssen WGM, Harder NM, Banck MS, Morrison JH, Beutler AS (2008) Sensory neuron targeting by self – complementary AAV8 via lumbar puncture for chronic pain. Proc Natl Acad Sci U S A 105:1055 – 1060.Su TP, Hayashi T, Maurice T, Buch S, Ruoho AE (2010) The sigma – 1 receptor chaperone as an inter – organelle signaling modulator. Trends Pharmacol Sci 31:557 – 566.Su TP, Hayashi T, Maurice T, Buch S, Ruoho AE (2010) The sigma – 1 receptor chaperone as an inter – organelle signaling modulator. Trends Pharmacol Sci 31:557 – 566.Su TP, Su TC, Nakamura Y, Tsai SY (2016) The Sigma – 1 receptor as a pluripotent modulator in living systems. Trends Pharmacol Sci 37:262 – 278.Su TP, Su TC, Nakamura Y, Tsai SY (2016) The Sigma – 1 receptor as a pluripotent modulator in living systems. Trends Pharmacol Sci 37:262 – 278.Suzuki T, Higgins PJ, Crawford DR (2000) Control selection for RNA quantitation. Biotechniques 29:332 – 337.Suzuki T, Higgins PJ, Crawford DR (2000) Control selection for RNA quantitation. Biotechniques 29:332 – 337.Tsai SY, Rothman RK, Su TP (2012) Insights into the sigma – 1 receptor chaperone’s cellular functions: a microarray report. Synapse 66:42 – 51.Tsai SY, Rothman RK, Su TP (2012) Insights into the sigma – 1 receptor chaperone’s cellular functions: a microarray report. Synapse 66:42 – 51.Tsai SY, Pokrass MJ, Klauer NR, Nohara H, Su TP (2015a) Sigma – 1 receptor regulates Tau phosphorylation and axon extension by shaping p35 turnover via myristic acid. Proc Natl Acad Sci U SA 112:6742 – 6747.Tsai SY, Pokrass MJ, Klauer NR, Nohara H, Su TP (2015a) Sigma – 1 receptor regulates Tau phosphorylation and axon extension by shaping p35 turnover via myristic acid. Proc Natl Acad Sci U SA 112:6742 – 6747.Tsai SY, Chuang JY, Tsai MS, Wang XF, Xi ZX, Hung JJ, Chang WC, Bonci A, Su TP (2015b) Sigma – 1 receptor mediates cocaineinduced transcriptional regulation by recruiting chromatinremodeling factors at the nuclear envelope. Proc Natl Acad Sci U S A 112:E6562 – E6570.Tsai SY, Chuang JY, Tsai MS, Wang XF, Xi ZX, Hung JJ, Chang WC, Bonci A, Su TP (2015b) Sigma – 1 receptor mediates cocaineinduced transcriptional regulation by recruiting chromatinremodeling factors at the nuclear envelope. Proc Natl Acad Sci U S A 112:E6562 – E6570.Wilson C, Gonza´ lez – Billault C (2015) Regulation of cytoskeletal dynamics by redox signaling and oxidative stress: implications for neuronal development and trafficking. Front Cell Neurosci 9:381.Wilson C, Gonza´ lez – Billault C (2015) Regulation of cytoskeletal dynamics by redox signaling and oxidative stress: implications for neuronal development and trafficking. Front Cell Neurosci 9:381.Xiao HS, Huang QH, Zhang FX, Bao L, Lu YJ, Guo C, Yang L, Huang WJ, Fu G, Xu SH, Cheng XP, Yan Q, Zhu ZD, Zhang X, Chen Z, Han ZG, Zhang X (2002) Identification of gene expression profile of dorsal root ganglion in the rat peripheral axotomy model of neuropathic pain. Proc Natl Acad Sci U S A 99:8360 – 8365.Xiao HS, Huang QH, Zhang FX, Bao L, Lu YJ, Guo C, Yang L, Huang WJ, Fu G, Xu SH, Cheng XP, Yan Q, Zhu ZD, Zhang X, Chen Z, Han ZG, Zhang X (2002) Identification of gene expression profile of dorsal root ganglion in the rat peripheral axotomy model of neuropathic pain. Proc Natl Acad Sci U S A 99:8360 – 8365.Zamanillo D, Romero L, Merlos M, Vela JM (2013) Sigma 1 receptor: a new therapeutic target for pain. Eur J Pharmacol 716:78 – 93.Zamanillo D, Romero L, Merlos M, Vela JM (2013) Sigma 1 receptor: a new therapeutic target for pain. Eur J Pharmacol 716:78 – 93.

The authors declare that there are no conflicts of interest present.